Baird-Parker Medium (BAM M17)

[所属分类:培养基配方] [发布时间:2019-12-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Baird-Parker Medium
(BAM M17)

培养基配方
Composition per liter:
Agar .....................................................20.0g
Glycine...................................................12.0g
Sodium pyruvate...........................................10.0g
Pancreatic digest of casein...............................10.0g
Beef extract...............................................5.0g
LiCl·6H2O.................................................5.0g
Yeast extract..............................................1.0g
Egg yolk tellurite enrichment ...........................50.0mL
pH 7.0 ± 0.2 at 25°C
Egg Yolk Tellurite Enrichment:
Composition per 100.0mL :
Chicken egg yolks...........................................10
K2TeO3 ..................................................0.15g
NaCl(0.9% solution).....................................50.0mL
Preparation of Egg Yolk Tellurite Enrichment:  Soak eggs with
1:100 dilution of saturated mercuric chloride solution for 1 min. Crack
11 eggs and separate yolks from whites. Mix egg yolks. Measure
30.0mL of egg yolk emulsion and add to 70.0mL of 0.9% NaCl solu-
tion. Mix thoroughly. Add 0.15g K2 TeO3 . Filter sterilize. Warm to 45°–
50°C.
Caution: Potassium tellurite is toxic.
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except EY tellurite en-
richment, to distilled/deionized water and bring volume to 950.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 48°–50°C. Aseptically add 50.0mL
of sterile EY tellurite enrichment. Mix thoroughly. Pour into sterile Pe-
tri dishes. The medium must be densely opaque. Dry plates before use.
Plates can be stored for up to 5 days at 20–25°C before use.
Use: For the selective isolation and enumeration of coagulase-positive
staphylococci from foods.