Bacteroides nodosus Agar

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides nodosus Agar

培养基配方
Composition per liter:
Agar ...............................................................14.0g
Liver hydrolysate...................................................10.0g
Proteose peptone No.3...............................................10.0g
Trypsin 1:250.......................................................10.0g
NaCl.................................................................5.0g
Yeast extract........................................................2.0g
L -Cysteine·HCl solution...........................................2.5mL
pH 7.4 ± 0.2 at 25°C
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine HCl......................................................1.0g
Preparation of  L -Cysteine·HCl Solution:  Dissolve 1.0g of  L -
cysteine·HCl in distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize. Warm to 50°C.
Preparation of Medium: Add components, except agar and  L -
cysteine·HCl solution, to distilled/deionized water and bring volume to
997.5mL. Mix thoroughly. Adjust pH to 8.5. Gently heat and bring to
boiling. Boil for 5 min. Filter and allow to cool to 25°C. Adjust pH to
7.4. Add 14.0g of agar. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add
2.5mL of sterile  L -cysteine·HCl solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Bacteroides nodosus.