Bacteroides Medium

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides Medium

培养基配方
Composition per liter:
Pancreatic digest of casein...............................27.0g
Yeast extract.............................................3.0g
K2HPO4 ....................................................2.5g
K2CO3 .....................................................2.0g
NaCl.......................................................2.0g
Hemin solution...........................................10.0mL
Vitamin K 1 solution......................................0.2mL
Hemin Solution:
Composition per 100.0mL:
Hemin .....................................................1.0g
NaOH (1N solution).......................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Bacteroides asaccharolyticus and Bacte-
roides melaninogenicus.