Bacteroides vulgatus Medium (LMG Medium 204)

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides vulgatus Medium
(LMG Medium 204)

培养基配方
Composition per liter:
Special peptone..............................................23.0g
Agar ........................................................15.0g
Glucose ......................................................5.0g
NaCl..........................................................5.0g
Soluble starch................................................1.0g
Cysteine hydrochloride........................................0.3g
Horse blood, sterile defibrinated...........................50.0mL
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
950.0mL distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile
horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation and maintenance of Bacteroides vulgatus.