Bacteroides HiVeg Agar Base with Selective Supplement (BBE)

[所属分类:培养基配方] [发布时间:2019-12-4] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides HiVeg Agar Base
with Selective Supplement
(BBE)

培养基配方
Composition per liter:
Plant hydrolysate .......................................25.0g
Agar.....................................................15.0g
Papaic digest of soybean meal............................10.0g
NaCl......................................................5.0g
Synthetic detergent No.II.................................2.0g
Esculin ..................................................1.0g
Ferric ammonium citrate...................................0.5g
Fe4(P2O7)3·H2O .........................................0.01g
Vitamin K 1 .............................................0.01g
Selective supplement solution ..........................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without selective supplement, is available as a
premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Gentamicin.................................................0.1mg
Preparation of Selective Supplement Solution: Add gentami-
cin to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium:  Add components, except selective sup-
plement, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Heat with frequent agitation and boil for 1 min to
completely dissolve. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Add 10.0mL of sterile selective supplement. Mix
thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the selection and presumptive identification of the Bacteri-
odes fragilis group. For the differentiation of Bacteroides species
based on the hydrolysis of esculin and presence of catalase. After incu-
bation for 48 hr, bacteria of the Bacteroides fragilis group appear as
gray, circular, raised colonies larger than 1.0mm. Esculin hydrolysis is
indicated by the presence of a blackened zone around the colonies.