APRY Broth Base with Chloramphenicol

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

APRY Broth Base with Chloramphenicol
Composition per liter:
Glucose .......................................................................................30.0g
Fructose.......................................................................................20.0g
Pancreatic digest of casein..........................................................15.0g
Polysorbate 80.............................................................................10.0g
Peptic digest of animal tissue........................................................5.0g
Yeast extract..................................................................................2.5g
Choramphenicol solution............................................................2.0ml
pH 6.5 ± 0.2 at 25°C
Source: This medium without chloramphenicol is available as a pre-
mixed powder from HiMedia. Chloramphenicol supplement is avail-
able from HiMedia.
Chloramphenicol Solution:
Composition per 2.0mL:
Chloramphenicol......................................................................50.0mg
Ethanol.......................................................................................2.0mL
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to ethanol and bring volume to 2.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–
50°C. Aseptically add 2.0mL chloramphenicol soultion. Mix thor-
oughly. Distribute into sterile tubes or flasks.
Use: For the detection and isolation of acid resistant yeasts, Zygosac-
charomyces bailii and Zygosaccharomyces rouxii, in salads, sauces,
and dressings.