APRY Broth

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

APRY Broth
Composition per liter:
Glucose .......................................................................................30.0g
Fructose.......................................................................................20.0g
Casein enzymatic hydrolysate ....................................................15.0g
Yeast extract..................................................................................2.5g
Peptic digest of animal tissue........................................................5.0g
Polysorbate 80..........................................................................10.0mL
Acetic acid, glacial.....................................................................5.0mL
Selective supplement solution ...................................................5.0mL
Potassium sorbate solution.........................................................1.0mL
pH 6.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Potassium Sorbate Solution:
Composition per 10.0mL:
Potassium sorbate .........................................................................1.0g
Preparation of Potassium Sorbate Solution:  Add components
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Selective Supplement Solution:
Composition per 10.0mL:
Chlortetracycline......................................................................50.0mg
Chloramphenicol......................................................................50.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except acetic acid, se-
lective supplement solution, and potassium sorbate solution, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add acetic acid, selective supplement solution, and potassium sor-
bate solution. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use: For the cultivation of acid resistant yeasts, including Zygosac-
charomyces bailii and Zygosaccharomyces rouxii n salads, sauces, and
dressings.