APRY Agar Base with Acetic Acid and Sorbate

[所属分类:培养基配方] [发布时间:2019-11-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

APRY Agar Base with Acetic Acid and Sorbate
Composition per liter:
Fructose.......................................................................................30.0g
NaCl............................................................................................25.0g
Glucose.......................................................................................20.0g
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue .......................................................5.0g
Yeast extract..................................................................................2.5g
Acetic acid (conc.).....................................................................5.0mL
Potassium sorbate (10%) ...........................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Source: This medium without acetic acid and potassium sorbate is
available as a premixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–
50°C. Aseptically add 5.0mL sterile acetic acid and 1.0mL potassium
sorbate solution. Mix thoroughly. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the detection and isolation of acid resistant yeasts, Zygosac-
charomyces bailii and Zygosaccharomyces rouxii, in salads, sauces,
and dressings.