培养基配方

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Urea Semisolid Medium

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A................................................... 400.0mL

Solution B ..................................................... 50.0mL

Solution A:

Composition per 400.0mL:

Pancreatic digest of casein..................................6.0g

Yeast extract........................................................2.0g

NaCl....................................................................1.0g

Yeast extract........................................................0.8g

Agar ....................................................................0.3g

L-Cystine .............................................................0.1g

Thioglycollic acid ......................................... 0.12mL

pH 7.2 ± 0.2 at 25°C

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

400.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 60°C.

Solution B:

Composition per 50.0mL:

Urea.....................................................................8.0g

Na2HPO4.............................................................3.8g

KH2PO4.............................................................3.64g

Yeast extract......................................................0.04g

Phenol Red.......................................................4.0mg

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to

50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine

400.0mL of sterile solution A and 50.0mL of sterile

solution B. Mix thoroughly. Aseptically distribute

into sterile screw-capped tubes in 7.0mL volumes.

Pass the tubes into an anaerobic chamber containing

85% N2 + 10% H2 + 5% CO2 for 60 min. Close screw

caps tightly.

Use: For the cultivation and differentiation of anaer

obic bacteria based on their production of urease.

Bacteria that produce urease turn the medium bright

red.