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Urea Test Broth

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Test Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea...................................................................20.0g

Na2HPO4.............................................................9.5g

KH2PO4...............................................................9.1g

Yeast extract........................................................0.1g

Phenol Red........................................................0.01g

Urea solution............................................... 100.0mL

Urea Solution:

Composition per 100.0mL:

Urea...................................................................20.0g

Preparation of Urea Solution: Add urea to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept urea solution, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile urea solution. Mix

thoroughly. Aseptically distribute into sterile tubes in

3.0mL volumes.

Use: For the cultivation and differentiation of mem

bers of the Enterobacteriaceae and aerobic actino

mycetes based on their production of urease. Bacteria

that produce urease turn the medium bright red.