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Urea Broth Base

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Broth Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................5.0g

Na2HPO4.............................................................1.2g

Peptone ...............................................................1.0g

Glucose ...............................................................1.0g

KH2PO4...............................................................0.8g

Phenol Red......................................................0.012g

Urea solution................................................. 50.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Urea Solution:

Composition per 100.0mL:

Urea...................................................................40.0g

Preparation of Urea Solution: Add urea to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept urea solution, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Auto

clave for 20 min at 10 psi pressure–115°C. Cool to

50°C. Aseptically add 50.0mL of sterile urea solu

tion. Mix thoroughly. Aseptically distribute into ster

ile tubes or flasks.

Use: For the differentiation of members of the Enter

obacteriaceae based on urease production. Urease

positive bacteria turn the medium pink.