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Urea Broth (Urease Test Broth) (BAM M171)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Broth

(Urease Test Broth)

(BAM M171)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea...................................................................20.0g

Na2HPO4.............................................................9.5g

KH2PO4...............................................................9.1g

Yeast extract........................................................0.1g

Phenol Red........................................................0.01g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostics.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Do nopt heat. Filter sterilize. Asep

tically distribute into sterile tubes or flasks.

Use: For the differentiation of organisms, especially

the Enterobacteriaceae, on the basis of urease produc

tion. Urease-positive bacteria turn the medium pink.