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Urea Agar (Urease Test Agar) (Urea Agar Base, Christensen)

[所属分类:培养基配方] [发布时间:2021-12-27] [发布人:网站管理员2] [阅读次数:] [返回]

Urea Agar

(Urease Test Agar)

(Urea Agar Base, Christensen)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Urea...................................................................20.0g

Agar ..................................................................15.0g

NaCl....................................................................5.0g

KH2PO4...............................................................2.0g

Peptone ...............................................................1.0g

Glucose ...............................................................1.0g

Phenol Red......................................................0.012g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept agar, to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Add agar to distilled/deionized water and bring vol

ume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°C. Aseptically add the

100.0mL of sterile basal medium. Mix thoroughly.

Distribute into sterile tubes. Allow tubes to solidify

in a slanted position.

Use: For the differentiation of a variety of microor

ganisms, especially members of the Enterobacteri

aceae, aerobic actinomycetes, streptococci, and

nonfermenting Gram-negative bacteria, on the basis

of urease production.