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培养基配方

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TYX Medium

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

TYX Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract........................................................5.0g

K2HPO4 ..............................................................3.5g

Tryptone..............................................................1.0g

Na2SO4 ...............................................................0.2g

L-cysteinium chloride·H2O .................................0.5g

Biotin .............................................................10.0mg

p-Aminobenzoic acid.....................................10.0mg

Sugar solution ................................................. 5.0mL

Resazurin ........................................................ 4.0mL

Trace elements solution .................................. 1.0mL

Resazurin Solution:

Composition per 100.0mL:

Resazurin ..........................................................25mg

Preparation of Resazurin Solution: Add resa

zurin to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per 100.0mL:

FeCl3·6H2O.........................................................2.7g

MgSO4 ................................................................1.2g

NaMoO4·2H2O .................................................0.24g

MnSO4·7H2O....................................................0.17g

CaCl2·2H2O ......................................................0.15g

ZnSO4·7H2O..................................................29.0mg

CuSO4·5H2O..................................................25.0mg

CoCl2·6H2O...................................................24.0mg

H2SO4 ............................................................. 2.8mL

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly.

Sugar Solution:

Composition per 100.0mL:

Xylose.................................................................1.0g

Preparation of Resazurin Solution: Add xy

lose to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept L-cysteinium chloride, biotin, p-aminobenzoic

acid, and sugar solution, to distilled/deionized water

and bring volume to 995.0mL. Mix thoroughly. Ad

just pH to 7.0. Gently heat but do not boil. Cook for

5-10 min so that color first turns red and then turns

yellow. Cool on ice. Add L-cysteinium chloride, bi

otin, and p-aminobenzoic acid. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C.

Aseptically add 5.0mL of sterile sugar solution. Mix

thoroughly.

Use: For the cultivation of Clostridium species.

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TYX Medium