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Tyrosine Agar (BAM M170)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

Tyrosine Agar

(BAM M170)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100mL:

Base solution...................................................... 1.0L

Tyrosine suspension.................................... 100.0mL

pH 7.0 ± 0.2 at 25°C

Base Solution:

Composition per 1.0L:

Agar ..................................................................15.0g

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Preparation of Base Solution: Add components

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 48°C.

Tyrosine Suspension:

Composition per 100.0mL:

Tyrosine ..............................................................5.0g

Preparation of Tyrosine Suspension: Add ty

rosine to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 48°C.

Preparation of Medium: Combine 1.0L sterile

base solution with 100.0mL sterile tyrosine suspen

sion. Mix thoroughly by swirling or inverting several

times. Distribute into sterile tubes or pour into sterile

Petri dishes. Cool rapidly to prevent tyrosine from

separating. For slants allow tubes to cool in an in

clined position.

Use: For the selective isolation of Brevibacillus brevis

and Bacillus cereus.