培养基配方

您现在的位置：网站首页 >> 培养基配方

UBA Medium (Universal Beer Agar)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

UBA Medium

(Universal Beer Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................16.1g

Peptonized milk ................................................15.0g

Agar ..................................................................12.0g

Tomato juice, dessicated...................................12.2g

Yeast extract........................................................6.1g

K2HPO4.............................................................0.31g

KH2PO4.............................................................0.31g

MgSO4·7H2O....................................................0.12g

FeSO4...............................................................6.0mg

MnSO4·5H2O...................................................6.0mg

NaCl.................................................................6.0mg

Beer............................................................. 250.0mL

pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components, except

beer, to distilled/deionized water and bring volume to

750.0mL. Mix thoroughly. Gently heat and bring to boil

ing. Add beer. Mix thoroughly. Distribute into tubes or

flasks. Autoclave for 10 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For cultivation of microorganisms of signifi

cance in the brewing industry.