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TYGS Medium (Tryptone Yeast Extract Glucose Salt Medium)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

TYGS Medium

(Tryptone Yeast Extract

Glucose Salt Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

NaCl....................................................................8.0g

Yeast extract........................................................1.0g

CaCl2·2H2O solution................................... 100.0mL

Glucose solution ......................................... 100.0mL

CaCl2·2H2O Solution:

Composition per 100.0mL:

CaCl2·2H2O ........................................................0.3g

Preparation of CaCl2·2H2O Solution: Add the

CaCl2·2H2O to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Glucose Solution:

Composition per 100.0mL:

D-Glucose............................................................1.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept CaCl2·2H2O solution and glucose solution, to

distilled/deionized water and bring volume to

800.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add the sterile

CaCl2·2H2O solution and sterile glucose solution.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation and maintenance of a vari

ety of bacteria.