培养基配方

您现在的位置：网站首页 >> 培养基配方

PE2 Medium

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

PE2 Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................20.0g

Yeast extract........................................................3.0g

Alaska seed peas ..........................................416–520

Bromcresol Purple solution ............................ 2.0mL

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ..............................................2.0g

Ethanol.......................................................... 10.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to 10.0mL of ethanol. Mix

thoroughly. Bring volume to 100.0mL with distilled/

deionized water. Filter sterilize.

Preparation of Medium: Add components, ex

cept Alaska seed peas, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat until dissolved. Add 8–10 Alaska seed peas to

each of 18 × 150 mm screw-capped tubes. Distribute

the broth into each tube in 19.0mL volumes. Allow

tubes to stand for 1 hr. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of Clostridium botulinum

from foods.