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Pectin Medium

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Pectin Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pectin ................................................................30.0g

Yeast extract........................................................5.0g

Bromthymol Blue (0.1% solution).................. 1.0mL

CaCl2·2H2O (10.0% solution)......................... 0.6mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add 100.0mL of dis

tilled/deionized water to a 2.0L flask and place on a

magnetic stirrer with no heat. While stirring, slowly

add CaCl2·2H2O, Bromthymol Blue solution, yeast

extract, and pectin. Add slowly to ensure each parti

cle is wetted. Gently heat and bring to almost boiling.

Adjust pH to 7.3 with 1N NaOH. Do not overshoot

pH 7.3.

Use: For the isolation and presumptive identification

of Yersinia enterocolitica and Yersinia pseudotuber

culosis from other fermenting Gram-negative bacilli

such as Enterocolitica agglomerans which is often

confused with Yersinia. Enterocolitica agglomerans

does not produce pectinase. Yersinia enterocolitica is

strongly positive for pectinase activity. Yersinia

pseudotuberculosis is weakly positive for pectinase

activity. Yersinia pestis is negative for pectinase ac

tivity. Also used for the differentiation of Klebsiella

oxytoca, which is pectinase positive.