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培养基配方

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Park and Sanders Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Basal medium .................................................... 1.0L

Supplement A ................................................. 5.0mL

Supplement B.................................................. 5.0mL

pH 7.0 ± 0.2 at 25°C

Basal Medium:

Composition per liter:

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue ...........................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

Sodium pyruvate...............................................0.25g

NaHSO3 ..............................................................0.1g

Horse blood, lysed ........................................ 50.0mL

Preparation of Basal Medium: Add compo

nents, except horse blood, to distilled/deionized wa

ter and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 25°C. Asepti

cally add sterile horse blood. Mix thoroughly.

Supplement A:

Composition per 5.0mL:

Vancomycin ......................................................0.01g

Trimethoprim lactate.........................................0.01g

Preparation of Supplement A: Add compo

nents to distilled/deionized water and bring volume

to 5.0mL. Mix thoroughly. Filter sterilize.

Supplement B:

Composition per 5.0mL:

Cefoperazone ..................................................0.032g

Cycloheximide....................................................0.1g

Preparation of Supplement B: Add components

to distilled/deionized water and bring volume to

5.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 1.0L of cooled, ster

ile basal medium, aseptically add 5.0mL of sterile

supplement A. Mix thoroughly. Aseptically distrib

ute into flasks in 100.0mL volumes. Inoculate medi

um with food samples. Incubate at 31°–32°C for 4 hr

to recover and resuscitate injured cells. Aseptically

add 0.5mL of supplement B to each 100.0mL of me

dium. Incubate cultures at 37°C for 2 hr.

Use: For the cultivation and enrichment of Campy

lobacter species from foods.

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Park and Sanders Enrichment Broth