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M17 Agar (LMG Medium 261)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Agar

(LMG Medium 261)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Disodium β-glycerophosphate..........................19.0g

Agar ..................................................................11.0g

Polypeptone ........................................................5.0g

Beef extract.........................................................5.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

Lactose solution ............................................ 50.0mL

MgSO4·7H2O (1M solution) ........................... 1.0mL

pH 6.9 ± 0.2 at 25°C

Lactose Solution:

Composition per 100.0mL:

Lactose..............................................................10.0g

Preparation of Lactose Solution: Add lactose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Preparation of Medium: Add components, ex

cept lactose solution, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat until boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile lactose solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation of Streptococcus thermophi

lus and for the cultivation and maintenance of strep

tococci and their bacteriophages. Also used for the

cultivation and maintenance of starter cultures for

cheese and yogurt manufacture as well as detecting

streptococcal mutants that are unable to ferment lac

tose.