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M16 Agar

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

M16 Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Beef extract.........................................................5.0g

Pancreatic digest of soybean meal......................5.0g

Polypeptone ........................................................5.0g

Sodium acetate·3H2O..........................................3.0g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

Carbohydrate solution................................... 50.0mL

pH 7.2 ± 0.2 at 25°C

Carbohydrate Solution:

Composition per 50.0mL:

Carbohydrate.......................................................5.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 50.0mL. Lactose or glucose may be used.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Adjust pH to 7.2

with 2N NaOH. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile carbohydrate solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the cultivation of lactobacilli from cheddar

cheese.