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M17 Agar

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

M17 Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Disodium β-glycerophosphate..........................19.0g

Agar ..................................................................11.0g

Beef extract.........................................................5.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................2.5g

Ascorbic acid ......................................................0.5g

MgSO4·7H2O ....................................................0.25g

Lactose solution ............................................ 50.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Lactose Solution:

Composition per 100.0mL:

Lactose..............................................................10.0g

Preparation of Lactose Solution: Add lactose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Preparation of Medium: Add components, ex

cept lactose solution, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat until boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL of sterile lactose solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and maintenance of strepto

cocci and their bacteriophages. Also used for the cul

tivation and maintenance of starter cultures for

cheese and yogurt manufacture as well as detecting

streptococcal mutants which are unable to ferment

lactose. For improved growth of lactic streptococci

and their bacteriophages and selective enumeration

of Streptococcus thermophilus from yogurt.