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Lysozyme Broth (BAM M90)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Lysozyme Broth

(BAM M90)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Nutrient broth..................................................... 1.0L

Lysozyme solution ........................................ 10.0mL

pH 6.9 ± 0.2 at 25°C

Nutrient Broth:

Composition per liter:

Pancreatic digest of gelatin.................................5.0g

Beef extract.........................................................3.0g

Source: Nutrient broth is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Nutrient Broth: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Distribute into bottles in

99.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C.

Lysozyme Solution:

Composition per 100.0mL:

Lysozyme............................................................0.1g

Preparation of Lysozyme Solution: Add

lysozyme to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add 1.0mL of sterile

lysozyme solution to 99.0mL of cooled, sterile nutri

ent broth. Mix thoroughly. Aseptically distribute into

sterile tubes in 2.5mL volumes.

Use: For the cultivation and differentiation of Bacil

lus cereus in foods. Bacillus cereus is resistant to

lysozyme and will grow in this medium.