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Lysine Ornithine Mannitol Agar (LOM Agar)

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Ornithine Mannitol Agar

(LOM Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

L–Ornithine·HCl .................................................6.5g

D–Mannitol .......................................................5.25g

L–Lysine·HCl ......................................................5.0g

NaCl....................................................................5.0g

Yeast extract........................................................3.0g

Bromthymol Blue ...............................................0.3g

Vancomycin solution..................................... 10.0mL

pH 6.5 ± 0.2 at 25°C

Vancomycin Solution:

Composition per 10.0mL:

Vancomycin·HCl...............................................0.03g

Preparation of Vancomycin Solution: Add

vancomycin to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept vancomycin solution, to distilled/deionized wa

ter and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile vancomycin solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and differentiation of Gram

negative bacilli based on their ability to decarboxy

late lysine or ornithine and mannitol fermentation.

Especially useful for the identification of Entero

bacter agglomerans. Bacteria that ferment mannitol

appear as dark yellow colonies. Bacteria that decar

boxylate lysine or ornithine appear as green-yellow

colonies.