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Lysine Medium

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

Lysine Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................44.5g

Agar ..................................................................17.8g

KH2PO4.............................................................1.78g

Lysine..................................................................1.0g

MgSO4·7H2O....................................................0.89g

CaCl2·2H2O ....................................................0.178g

NaCl................................................................0.089g

Inositol ..............................................................0.02g

Calcium pantothenate ......................................2.0mg

Adenine..........................................................1.78mg

DL-Methionine................................................0.89mg

L-Histidine......................................................0.89mg

DL-Tryptophan................................................0.89mg

Thiamine·HCl ..................................................0.4mg

Pyridoxine........................................................0.4mg

Nicotinic acid...................................................0.4mg

FeSO4·7H2O...................................................0.22mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

MnSO4·H2O.................................................0.035mg

ZnSO4·7H2O ................................................0.035mg

(NH4)2MoO4·4H2O......................................0.018mg

H3BO3 ...............................................................8.9μg

Biotin ................................................................2.0μg

Folic acid ..........................................................1.0μg

Potassium lactate (50% solution).................. 10.0mL

Lactic acid....................................................... 1.0mL

pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add potassium lactate

to distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Add remaining compo

nents, except lactic acid. Mix thoroughly. Gently heat

while stirring and bring to boiling. Do not autoclave.

Cool to 50°C. Adjust pH to 4.8 by adding 1.0mL of

lactic acid. Pour into sterile Petri dishes. Dry the sur

face of the plates by incubation at 37°C for 24 hr.

Use: For the isolation, cultivation, and enumeration

of wild yeasts encountered in brewing. For the isola

tion and enumeration of wild yeasts encountered in

brewing. On this medium, pitching yeasts are sup

pressed.

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Lysine Medium