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培养基配方

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Lowenstein-Jensen Medium

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1600.0mL:

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate...............................................0.6g

Malachite Green..................................................0.4g

MgSO4·7H2O ....................................................0.24g

Homogenized whole egg .................................. 1.0L

Glycerol ........................................................ 12.0mL

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems and Oxoid.

Homogenized Whole Egg:

Composition per liter:

Whole eggs ...................................................... 18-24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except fresh egg

mixture. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 1.0L

of homogenized whole egg. Mix thoroughly. Distrib

ute into sterile screw-capped tubes. Place tubes in a

slanted position. Inspissate at 85°C (moist heat) for

45 min.

Use: For the cultivation and differentiation of Myco

bacterium species. Mycobacterium tuberculosis ap

pears as granular, rough, dry colonies. Mycobacterium

kansasii appears as smooth to rough photochromoge

nic colonies. Mycobacterium gordonae appears as

smooth yellow-orange colonies. Mycobacterium avi

um appears as smooth, colorless colonies. Mycobacte

rium smegmatis appears as wrinkled, creamy white

colonies. Also used for the cultivation and mainte

nance of Gordona species, Nocardia species, Rhodo

coccus species, and Tsukamurella paurometabolum.

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Lowenstein-Jensen Medium