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Lowenstein-Jensen Medium with NaCl

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1600.0mL:

NaCl..................................................................80.0g

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate ..............................................0.6g

Malachite Green..................................................0.4g

MgSO4·7H2O....................................................0.24g

Homogenized whole egg .................................. 1.0L

Glycerol ........................................................ 12.0mL

Homogenized Whole Egg:

Composition per liter:

Whole eggs ...................................................... 18-24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thoroughly.

Add remaining components, except fresh egg mixture.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 1.0L of homog

enized whole egg. Mix thoroughly. Distribute into

sterile screw-capped tubes. Place tubes in a slanted po

sition. Inspissate at 85°C (moist heat) for 45 min.

Use: For the cultivation of Mycobacterium smegma

tis and other salt-tolerant Mycobacterium species.