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培养基配方

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Lowenstein-Gruft Medium

[所属分类:培养基配方] [发布时间:2021-11-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1600.0mL:

Potato starch......................................................30.0g

Asparagine ..........................................................3.6g

KH2PO4...............................................................2.4g

Magnesium citrate ..............................................0.6g

Malachite Green..................................................0.4g

MgSO4·7H2O....................................................0.24g

Nalidixic acid..................................................0.056g

Ribonucleic acid ............................................0.08mg

Homogenized whole egg .................................. 1.0L

Glycerol ........................................................ 12.0mL

Penicillin......................................................80,000U

Homogenized Whole Egg:

Composition per liter:

Whole eggs ......................................................18–24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for

15 min. Break the eggs into a sterile container. Ho

mogenize by shaking. Filter through four layers of

sterile cheesecloth into a sterile graduated cylinder.

Measure out 1.0L.

Preparation of Medium: Add glycerol to

600.0mL of distilled/deionized water. Mix thorough

ly. Add remaining components, except fresh egg

mixture. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 1.0L

of homogenized whole egg. Mix thoroughly. Distrib

ute into sterile screw-capped tubes. Place tubes in a

slanted position. Inspissate at 85°C (moist heat) for

45 min.

Use: For the cultivation and differentiation of Myco

bacterium species. Mycobacterium tuberculosis ap-

pears as granular, rough, dry colonies. Mycobacterium

kansasii appears as smooth to rough photochromoge

nic colonies. Mycobacterium gordonae appears as

smooth yellow-orange colonies. Mycobacterium avi

um appears as smooth, colorless colonies. Mycobacte

rium smegmatis appears as wrinkled, creamy white

colonies.

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Lowenstein-Gruft Medium