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Listeria Fermentation Broth

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3 .....................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Bromcresol Purple ..............................................0.1g

Carbohydrate solution................................... 10.0mL

Carbohydrate Solution:

Composition per 10.0mL:

Carbohydrate.......................................................5.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use glucose, salicin, rhamnose, dulcitol, or raffinose.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile carbohydrate solution. Mix

thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation and differentiation of Liste

ria species based on the fermentation of glucose, sali

cin, rhamnose, dulcitol, and raffinose.