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Listeria Enrichment Broth II, USDA FSIS (Listeria Primary Selective Enrichment Broth, UVM II) (University of Vermont II Listeria Primary Selective Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Enrichment Broth II,

USDA FSIS

(Listeria Primary Selective

Enrichment Broth, UVM II)

(University of Vermont II Listeria

Primary Selective Enrichment Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Na2HPO4...........................................................12.0g

Beef extract.........................................................5.0g

Protease peptone .................................................5.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................5.0g

KH2PO4.............................................................1.35g

Esculin ................................................................1.0g

Nalidixic acid solution.................................... 1.0mL

Acriflavine solution ........................................ 1.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid......................................................0.2g

NaOH (0.1M solution) .................................. 10.0mL

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to 10.0mL of NaOH solution. Mix

thoroughly. Filter sterilize.

Acriflavine Solution:

Composition per 10.0mL:

Acriflavine ........................................................0.25g

Preparation of Acriflavine Solution: Add acri

flavine to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize. Use

freshly prepared solution.

Preparation of Medium: Add components, ex

cept nalidixic acid solution and acriflavine solution,

to distilled/deionized water and bring volume to

998.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile na

lidixic acid solution. Mix thoroughly. Store at 4°C.

Immediately prior to use, aseptically add 1.0mL of

sterile acriflavine solution. Mix thoroughly. Asepti

cally distribute into sterile tubes or flasks.

Use: For the selective isolation, cultivation, and en

richment of Listeria monocytogenes from food, milk

and dairy products.

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Listeria Enrichment Broth II, USDA FSIS (Listeria Primary Selective Enrichment Broth, UVM II) (University of Vermont II Listeria Primary Selective Enrichment Broth