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Listeria Transport Enrichment Medium

[所属分类:培养基配方] [发布时间:2021-11-26] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Transport

Enrichment Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glycerophosphate.................................10.0g

Agar ....................................................................2.0g

Sodium thioglycolate..........................................1.0g

CaCl2...................................................................0.1g

Nalidixic acid....................................................0.04g

Acridine solution ............................................ 2.0mL

pH 7.4 ± 0.2 at 25°C

Acridine Solution:

Composition per 10.0mL:

Acridine ............................................................0.04g

Preparation of Acridine Solution: Add acri

dine to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, ex

cept acridine solution, to distilled/deionized water

and bring volume to 998.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add acridine solution. Mix thoroughly. Aseptically

distribute into sterile tubes in 10.0mL volumes or fill

bottles 4/5’s full.

Use: For the maintenance—as a transport medium—

and enrichment of Listeria species.