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Lactobacilli MRS Broth with Ethanol

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................5.0g

L-Cysteine ...........................................................2.0g

Ammonium citrate ..............................................2.0g

Na2HPO4 .............................................................2.0g

Tween 80.............................................................1.0g

MgSO4·7H2O ......................................................0.1g

MnSO4·5H2O....................................................0.05g

Ethanol (95% solution)............................... 100.0mL

pH5.0 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept ethanol, to distilled/deionized water and bring

volume to 990.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C. Filter sterilize etha

nol. Aseptically add 100.0mL of sterile ethanol. Mix

thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation and maintenance of Lacto

bacillus species.