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Lactobacillus bulgaricus Agar (LB Agar)

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

Lactobacillus bulgaricus Agar

(LB Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 900.0mL:

Agar ..................................................................20.0g

Glucose .............................................................20.0g

Beef extract.......................................................10.0g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................5.0g

K2HPO4...............................................................2.0g

Tween 80.............................................................1.0g

Acetate buffer ............................................... 80.0mL

Tomato juice, filtered.................................... 40.0mL

pH 6.8 ± 0.2 at 25°C

Acetate Buffer:

Composition per liter:

Sodium acetate..............................................113.55g

Acetic acid .................................................... 10.0mL

Preparation of Acetate Buffer: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, ex

cept acetate buffer, to distilled/deionized water and

bring volume to 820.0mL. Mix thoroughly. Gently

heat and bring to boiling. Adjust pH to 6.8. Add

80.0mL of acetate buffer. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes.

Use: For the isolation, cultivation, and enumeration

of Lactobacillus bulgaricus from foods.