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Lactobacilli MRS Broth with Cysteine

[所属分类:培养基配方] [发布时间:2021-11-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

Sodium acetate....................................................5.0g

Yeast extract........................................................5.0g

L-Cysteine ...........................................................2.0g

Ammonium citrate ..............................................2.0g

Na2HPO4 .............................................................2.0g

Tween 80.............................................................1.0g

MgSO4·7H2O ......................................................0.1g

MnSO4·5H2O ....................................................0.05g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Lactoba

cillus species, including L. ruminis and L. vitulinus.