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Korthof Medium

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

Korthof Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1088.0mL:

NaCl....................................................................1.4g

Na2HPO4·2H2O.................................................0.88g

Peptone ...............................................................0.8g

KH2PO4.............................................................0.24g

CaCl2.................................................................0.04g

KCl....................................................................0.04g

NaHCO3............................................................0.02g

Rabbit serum, inactivated ............................. 80.0mL

Rabbit hemoglobin solution............................ 8.0mL

pH 7.2 ± 0.2 at 25°C

Rabbit Hemoglobin Solution:

Composition per 20.0mL:

Rabbit blood clot........................................... 10.0mL

Preparation of Rabbit Hemoglobin Solution:

Add rabbit blood clot to 10.0mL of distilled/deionized

water. Lyse the clot by freezing and thawing.

Preparation of Medium: Add components, ex

cept rabbit serum and rabbit hemoglobin solution, to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling. Cool

to 25°C. Filter through Whatman #1 filter paper. Dis

tribute into flasks in 100.0mL volumes. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 8.0mL of rabbit serum and 0.8mL of

rabbit hemoglobin solution to each flask. Mix thor

oughly.

Use: For the cultivation of Leptospira species.