培养基配方

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Korthof Medium, Modified

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl....................................................................1.4g

Na2HPO4·2H2O.................................................0.88g

Peptone................................................................0.8g

KH2PO4.............................................................0.24g

CaCl2.................................................................0.04g

KCl....................................................................0.04g

NaHCO3 ............................................................0.02g

Rabbit serum, heat inactivated at 56°C....... 100.0mL

pH 7.2–7.6 at 25°C

Preparation of Medium: Add components, ex

cept rabbit serum, to distilled/deionized water and

bring volume to 900.0L. Mix thoroughly. Gently heat

and bring to boiling. Boil for 20 min. Cool overnight

at 4°C. Filter through Whatman #2 filter paper. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°–56°C. Aseptically

add 100.0mL of rabbit serum. Mix thoroughly.

Use: For the cultivation of Leptospira species.