培养基配方

您现在的位置：网站首页 >> 培养基配方

KM Agar (Kempler-McKay Agar)

[所属分类:培养基配方] [发布时间:2021-11-24] [发布人:网站管理员2] [阅读次数:] [返回]

KM Agar

(Kempler-McKay Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Milk, nonfat ......................................................10.0g

Glucose ...............................................................5.0g

Milk protein hydrolysate.....................................2.5g

Solution 1...................................................... 10.0mL

Solution 2...................................................... 10.0mL

pH 6.6 ± 0.2 at 25°C

Solution 1:

Composition per 100.0mL:

K3Fe(CN)6 ........................................................10.0g

Preparation of Solution 1: Add components to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Solution 2:

Composition per 40.0mL:

Ferric citrate........................................................1.0g

Sodium citrate.....................................................1.0g

Preparation of Solution 2: Add components to

distilled/deionized water and bring volume to 40.0mL.

Mix thoroughly. Filter sterilize.

Caution: Cyanide is toxic.

Preparation of Medium: Add components, ex

cept solution 1 and solution 2, to distilled/deionized

water and bring volume to 980.0mL. Mix thoroughly.

Gently heat and bring to boiling. Adjust pH to 6.6. Au

toclave for 12 min at 10 psi pressure–115°C. Cool to

45°–50°C. Aseptically add sterile solution 1 and solu

tion 2. Mix thoroughly. Pour into sterile Petri dishes.

Allow plates to dry in the dark at 30°C for 24 hr before

using.

Use: For the isolation and cultivation of acidogenic

microorganisms from foods. For the differentiation

of citrate-fermenting lactic bacteria, such as Lacto

bacillus lactis subspecies diacetylactis, from the

noncitrate-fermenting Lactobacillus lactis subspe

cies cremoris.