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HiCrome OGYE Agar Base (OGYE Agar Base HiCrome) (Oxytetracyclin Glucose Yeast Extract Agar HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome OGYE Agar Base

(OGYE Agar Base HiCrome)

(Oxytetracyclin Glucose

Yeast Extract Agar HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ............................................................20.0g

Agar ..................................................................12.0g

Yeast extract .......................................................4.0g

Chromogenic mixture ........................................1.1g

Oxytetracycline selective supplement .......... 10.0mL

pH 7.0 ± 0.2 at 25°C

Oxytetracylcine Selective Supplement:

Composition per 10.0mL:

Oxytetracycline...................................................0.1g

Preparation of Oxytetracylcine Selective

Supplement: Add 0.1g oxytetracyline to 10.0mL

of distilled/deionized water. Mix thoroughly. Filter

sterilize.

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components, ex

cept oxytetracycline selective supplement, to dis

tilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 10.0mL sterile

oxytetracylcine selective supplement. Mix thorough

ly. Pour into sterile Petri dishes.

Use: For the rapid isolation of yeasts and molds from

milk and milk products. Oxytetracycline makes the

medium more selective by inhibiting the growth of

lactobacilli. Aspergillus niger appears as light blue

colored colonies with black spores due to presence of

chromogenic mixture, Candida albicans shows

green colored colonies and Saccharomyces cerevisi

ae gives colorless colonies.