培养基配方

您现在的位置：网站首页 >> 培养基配方

HiCrome Rapid Coliform Broth (Coliform Rapid HiCrome Broth) (Rapid Coliform HiCrome Broth)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome Rapid Coliform Broth

(Coliform Rapid HiCrome Broth)

(Rapid Coliform HiCrome Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone, special ..................................................5.0g

NaCl....................................................................5.0g

Na2HPO4.............................................................2.7g

KH2PO4...............................................................2.0g

Sorbitol ...............................................................1.0g

Sodium lauryl sulfate..........................................0.1g

IPTG....................................................................0.1g

Chromogenic substrate .....................................0.08g

Fluorogenic substrate........................................0.05g

pH 6.8 ± 0.3 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes.

Use: For the detection and conformation of Escher

ichia coli and coliforms on the basis of enzyme sub

strate reaction from water samples, using a combina

tion of chromogenic and fluorogenic substrate.