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HiCrome MS.O157 Agar with Tellurite (MS.O157 Agar with Tellurite, HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome MS.O157 Agar

with Tellurite

(MS.O157 Agar

with Tellurite, HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition 1000.25mL:

Agar ..................................................................12.0g

Peptone, special ................................................10.0g

Sorbitol................................................................4.0g

Bile salt mixture..................................................1.0g

Chromogenic mixture .....................................0.731g

Tellurite solution ........................................... 0.25mL

pH 6.8 ± 0.2 at 25°C

Tellurite Solution:

Composition per 10.0mL:

K2TeO3 ................................................................0.1g

Preparation of Tellurite Solution: Add compo

nents to 10.0mL of distilled/deionized water. Mix

thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components, ex

cept tellurite solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

while stirring and bring to boiling. Do not autoclave.

Cool to 45°C. Aseptically add 0.25mL sterile tellurite

solution. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the simultaneous detection of Escherichia

coli, Escherichia coli O157:H7, and coliforms in wa

ter and food samples. Escherichia coli O157:H7 gives

colorless colonies because of non-fermentation of sor

bitol and absence of β-glucuronidase activity, whereas

other strains of Escherichia coli having β-glucu

ronidase activity and fermenting sorbitol appear as

steel blue colored colonies. Addition of tellurite makes

the medium much more specific and selective.