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HiCrome MS.O157 Agar (MS.O157 Agar HiCrome)

[所属分类:培养基配方] [发布时间:2021-11-23] [发布人:网站管理员2] [阅读次数:] [返回]

HiCrome MS.O157 Agar

(MS.O157 Agar HiCrome)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Peptone, special ................................................10.0g

Sorbitol ...............................................................4.0g

Bile salt mixture..................................................1.0g

Chromogenic mixture .....................................0.731g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sig

ma-Aldrich.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Do not autoclave. Cool to 50°C. Pour into

sterile Petri dishes.

Use: For the simultaneous detection of Escherichia

coli, Escherichia coli O157:H7, and coliforms in wa

ter and food samples. Escherichia coli O157 gives

colorless colonies because of non-fermentation of

sorbitol and absence of β-glucuronidase activity,

whereas other strains of Escherichia coli having β-

glucuronidase activity and fermenting sorbitol ap

pear as steel blue colored colonies. Some non-Es

cherichia coli O157 may have some colony color.