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Fluid Thioglycolate Medium without Glucose or Eh Indicator

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

Fluid Thioglycolate Medium

without Glucose or Eh Indicator

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................... 20.0g

NaCl................................................................... 2.5g

Agar ................................................................. 0.75g

L-Cystine ............................................................ 0.5g

Sodium thioglycolate......................................... 0.5g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Aseptically distribute into

sterile screw-capped tubes or flasks.

Use: For cultivation of anaerobic bacteria. For use as

a basal medium in carbohydrate fermentation tests

for differentiating anaerobic bacteria. For carbohy

drate fermentation tests, 1.0mL of a 10% filter-steril

ized carbohydrate solution is aseptically added to

9.0mL of fluid thioglycolate medium.