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Fluorescent Pectolytic Agar (FPA Medium)

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

Fluorescent Pectolytic Agar

(FPA Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3.................................... 20.0g

Agar ................................................................. 15.0g

Pectin ................................................................. 5.0g

K2HPO4.............................................................. 1.5g

MgSO4·7H2O................................................... 0.73g

Antibiotic solution ....................................... 10.0mL

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide...............................................0.075g

Novobiocin ....................................................0.045g

Penicillin G ................................................. 75,000U

Ethanol........................................................... 1.0mL

Preparation of Antibiotic Solution: Add compo

nents to 1.0mL of ethanol. Mix thoroughly. Let stand

for 30 min. Bring volume to 10.0mL with distilled/

deionized water. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept antibiotic solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile antibiotic solution. Mix thor

oughly. Pour into sterile Petri dishes.

Use: For the cultivation of fluorescent Pseudomonas

species that are pectinolytic.