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Fluid Thioglycolate Medium with Rabbit Serum

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

Fluid Thioglycolate Medium

with Rabbit Serum

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein...............................15.0g

Glucose ..............................................................5.5g

Yeast extract.......................................................5.0g

NaCl...................................................................2.5g

Agar .................................................................0.75g

L-Cystine ............................................................0.5g

Sodium thioglycolate .........................................0.5g

Resazurin ........................................................1.0mg

Rabbit serum.............................................. 100.0mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept rabbit serum, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Aseptically add 100.0mL rabbit serum. Asep

tically distribute into sterile screw-capped tubes or

flasks.

Use: For cultivation of anaerobic bacteria. For use in

sterility testing.