培养基配方

您现在的位置：网站首页 >> 培养基配方

Fluid Thioglycolate Medium with K Agar

[所属分类:培养基配方] [发布时间:2021-11-19] [发布人:网站管理员2] [阅读次数:] [返回]

Fluid Thioglycolate Medium

with K Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein...............................15.0g

Glucose ..............................................................5.0g

Yeast extract.......................................................5.0g

KCl.....................................................................2.5g

K agar...............................................................0.45g

L-Cystine ............................................................0.5g

Resazurin ........................................................1.0mg

Thioglycollic acid .......................................... 0.3mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. If medium becomes oxi

dized before use (resazurin turns red), heat in a boil

ing water bath to expel absorbed O2. Cool to 25°C.

Use: For the cultivation of various microorganisms.

For use in sterility test.