培养基配方

您现在的位置：网站首页 >> 培养基配方

Fletcher Medium with Fluorouracil (Fluorouracil Leptospira Medium)

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Fletcher Medium with Fluorouracil

(Fluorouracil Leptospira Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ...................................................................1.5g

NaCl................................................................... 0.5g

Peptone .............................................................. 0.3g

Beef extract........................................................ 0.2g

Rabbit serum................................................ 50.0mL

Fluorouracil solution.................................... 20.0mL

pH 7.9 ± 0.1 at 25°C

Fluorouracil Solution:

Composition per 100.0mL:

Fluorouracil...................................................... 10.0g

Preparation of Fluorouracil Solution: Add

fluorouracil to 50.0mL of distilled/deionized water.

Add 1.0mL of 2N NaOH and bring volume to

100.0mL. Gently heat to 56°C for 2 hr. Adjust pH to

7.4–7.6 with NaOH. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept rabbit serum and fluorouracil solution, to dis

tilled/deionized water and bring volume to 930.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Aseptically add 80.0mL of sterile rab

bit serum. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks. Immediately prior to use, add

0.1mL of fluorouracil solution per 5.0mL of medium.

Use: For the isolation, cultivation, and maintenance

of cultures of Leptospira species.