培养基配方

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Fletcher’s Semisolid Medium

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 2120.0mL:

Agar ................................................................... 1.5g

NaCl................................................................... 0.5g

Peptone .............................................................. 0.3g

Beef extract........................................................ 0.2g

Rabbit serum.............................................. 240.0mL

pH 7.9 ± 0.1 at 25°C

Preparation of Medium: Add components, ex

cept rabbit serum, to distilled/deionized water and

bring volume to 1880.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°–55°C. Aseptically

add 240.0mL of sterile rabbit serum. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the isolation, cultivation, and maintenance

of cultures of Leptospira species.