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Fletcher Medium

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Fletcher Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ...................................................................1.5g

NaCl...................................................................0.5g

Peptone...............................................................0.3g

Beef extract........................................................0.2g

Rabbit serum................................................ 50.0mL

pH 7.9 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept rabbit serum, to distilled/deionized water and

bring volume to 950.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°–55°C. Aseptically

add 50.0mL of sterile rabbit serum. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the isolation, cultivation, and maintenance

of cultures of Leptospira species.