培养基配方

您现在的位置：网站首页 >> 培养基配方

Fluid Thioglycolate Agar with Calcium Carbonate

[所属分类:培养基配方] [发布时间:2021-11-18] [发布人:网站管理员2] [阅读次数:] [返回]

Fluid Thioglycolate Agar

with Calcium Carbonate

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar .................................................................75.0g

Pancreatic digest of casein...............................15.0g

CaCO3 ..............................................................10.0g

Glucose ..............................................................5.0g

Yeast extract.......................................................5.0g

NaCl...................................................................2.5g

L-Cystine ............................................................0.5g

Sodium thioglycolate .........................................0.5g

Resazurin .......................................................0.001g

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept CaCO3, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Sterilize

CaCO3 by autoclaving for 15 min at 15 psi pressure–

121°C. Aseptically add sterile CaCO3 to sterile tubes

or plates—0.1g of sterile CaCO3 per 10.0mL of me

dium to be added. Pour medium into sterile Petri

dishes or distribute into sterile tubes.

Use: For the rapid cultivation of anaerobic bacteria.

For the cultivation and maintenance of Campylo

bacter fetus, Campylobacter jejuni, and Streptococ

cus species.